synthesis, labeling and quality control of a new neuropeptide y analogue for diagnosis of breast tumors
نویسندگان
چکیده
introduction: over expression of selected peptide receptors in human tumors has been shown to represent clinically relevant targets for cancer diagnosis and therapy. the aim of this work was to investigate neuropeptide y (npy) as a new radiopharmaceutical for diagnosis of breast cancer. methods: a neuropeptide y analogues with y1 receptor preference and agonistic properties was synthesized by solid phase method. after conjugation with diethylenetriaminepentaacetic acid (dtpa) labeling with 111in was performed. for labeled peptide, yield of labeling, stability in human serum, receptor binding in cell surface with internalization in sk-n-mc cells, and biodistribution in normal rat were determined. results: peptide was synthesized and labeled with more than 95% purity. radiolabeled peptide was stable in human serum and specifically binds and internalized in the cells with y1 receptor (4h = 22%). a rapid clearance from blood pool and urinary with hepatobiliary excretion were observed. conclusion: our results showed that this peptide can be considered as a candidate for diagnosis of breast tumors
منابع مشابه
Synthesis, labeling and quality control of a new Neuropeptide Y analogue for diagnosis of breast tumors
Introduction: Over expression of selected peptide receptors in human tumors has been shown to represent clinically relevant targets for cancer diagnosis and therapy. The aim of this work was to investigate Neuropeptide Y (NPY) as a new radiopharmaceutical for diagnosis of breast cancer. Methods: A neuropeptide Y analogues with Y1 receptor preference and agonistic p...
متن کاملSynthesis, Labeling and Quality Control of a New Neuropeptide Y Analogue for Diagnosis of Breast Tumors
Introduction: Over expression of selected peptide receptors in human tumors has been shown to represent clinically relevant targets for cancer diagnosis and therapy. The aim of this work was to investigate Neuropeptide Y (NPY) as a new radiopharmaceutical for diagnosis of breast cancer. Methods: A neuropeptide Y analogues with Y1 receptor preference and agonistic properties was synthesized by s...
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Introduction: Bombesin (BN), a 14-amino acid neuropeptide, shows high affinity for the human GRP (gastrin releasing peptide) receptors, which are overexpressed by a variety of cancers, including prostate, breast, pancreas, gastrointestinal, and small cell lung cancer. Aim was to prepare [6-hydrazinopyridine-3-carboxylic acid (HYNIC0), D-Tyr6, D...
متن کاملsynthesis and evaluation of a new radiolabeled bombesin analogue for diagnosis of grp receptor expressing tumors
introduction: bombesin (bn), a 14-amino acid neuropeptide, shows high affinity for the human grp (gastrin releasing peptide) receptors, which are overexpressed by a variety of cancers, including prostate, breast, pancreas, gastrointestinal, and small cell lung cancer. aim was to prepare [6-hydrazinopyridine-3-carboxylic acid (hynic0), d-tyr6, d-trp8] - bn [6-14] nh2 that could be easily labeled...
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عنوان ژورنال:
iranian journal of nuclear medicineناشر: tehran university of medical sciences
ISSN 1681-2824
دوره 16
شماره 1 2008
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